I want to increase the lens magnification transfer large image resolution, but the image mask and the substrate can not at the same time clear, is there any way?

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Image Problems

Would using two benefits that the alignment of the lens?
When will a suitable alignment of the lens?
My quick scan station can not be good lock cylinder to move the lens holder when the lens position will shift about, what is there fault?
How to adjust the lens magnification should fit?
I want to increase the lens magnification transfer large image resolution, but the image mask and the substrate can not at the same time clear, is there any way?
Would using coaxial lighting timing of each of the annular lighting Why?

I want to increase the lens magnification transfer large image resolution, but the image mask and the substrate can not at the same time clear, is there any way?

Increase the depth of field image magnification image becomes small, a small adjustment to align gap to get a clear image of the mask and the substrate, but be careful to avoid align gap is too small to cause friction between the two contacts.